Elisa kits

Download our price list in pdf:ELISA kits full pricelist
Download our price list in ecxel:ELISA kits full pricelist

If you don't find your kit just give us an call or send an mail because we have over the 25000 elisa kits, We have everything you need for a good price

Information about Elisa

ELISA kit assay

Enzyme-Linked Immunosorbent Assays (ELISA) combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or antigens coupled to an easily assayed enzyme that possesses a high turnover number. ELISAs can provide a useful measurement of antigen or antibody concentration.

We offer a variety of analytically validated, preconfigured ELISA assays, reagents and accessories for the study of metabolic disease markers and other vital research targets.

Applications

Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV testor West Nile Virus). It has also found applications in the food industry in detecting potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs.

The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400-fold and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" — an antibody that binds to other antibodies — is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme. Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and negative result.

A cut-off point may be determined by comparing it with a known standard. If an ELISA test is used for drug screening at workplace, a cut-off concentration, 50 ng/mL, for example, is established, and a sample will be prepared which contains the standard concentration of analyte. Unknowns that generate a signal that is stronger than the known sample are "positive". Those that generate weaker signal are "negative."

For more information about Elisa (kits) just give us
an call: +32 16 58 90 45 or send an mail:[email protected]
More contact information on our Contact page

Our newest products - Kronos Dio, Bullet Blender, Targatt, PCR PreMix (Order now! Best Prices Online!)

Information

-->tel. +32 16 58 90 45 (Belgium)
-->mail:[email protected]
-->www.gentaur.com
-->more contact information

Promotions other products:

Dynabeads
MagSI-proteomics
MagSI
Recombinant Human Interleukin-4